How to ensure that cows of good quality from one generation to another, and rejecting inherited diseases for the next generation, is crucial for the detection of cows with recessive genetic defects. Therefore, this area has become a hot topic in the dairy industry in foreign countries in recent years. Chinese scientists and technicians have also done in-depth research work. The pastor Li Yanhua of the Beijing Dairy Cow Center recently published an article on the establishment of a method for detecting bovine leukocyte adhesion defect using single-strand conformation polymorphism in the journal “Heritage†and reported on her. The research results: Using PCR-specific primers designed by ourselves, through the screening and optimization of PCR, PCR-SSCP conditions, combined with DNA sequencing, established a new PCR-SSCP detection of harmful genes of bovine leukocyte adhesion deficiency (BLAD) method. The method is simple, quick, accurate, and has wide use samples. It is suitable for screening large samples.
In developed countries, foreign developed countries attach great importance to the detection of recessive genetic defect genes such as BLAD and CVM (vertebral deformity syndrome). All reserve bulls must pass these genetic tests to prove that they are not carriers of recessive harmful genes before they can enter into progeny determination procedures to do performance evaluation and promote the use of them, so as to ensure the genetic advantage of breeding bulls and reduce the spread of harmful genes. In our country, the detection and molecular breeding of recessive harmful genes such as BLAD and CVM have not yet been carried out in breeding breeding bulls, which is a potential risk in the development of China's dairy industry.
According to reports, Chinese researchers have developed a new method for detecting hidden and harmful genes in cows, which is a simple and quick method for detecting harmful genes such as BLAD and CVM. It is a kind of molecular genetic breeding and molecular breeding technology that uses molecular genetics and molecular biological technology to establish the detection of hidden harmful genes such as BLAD and CVM. The use of this technology can effectively reduce the transmission of herds of genetic diseases; further improve the breeding system of breeding bulls; and expand the genetic impact of excellent bulls. Experts believe that this research result provides a theoretical basis for molecular breeding of dairy cows in China. It provides new methods and ideas for the classification and screening of harmful genes in BLAD of dairy cows in China. It is very important for self-cultivation of excellent reserve bulls in China and perfecting the breeding system for dairy cows.